Ekstraksi DNA total dari sumber jaringan hewan (Ikan Kerapu) menggunakan metode kit for animal tissue

  • Yanti Ariyanti Program Studi Biologi, Jurusan Sains, Institut Teknologi Sumatera, Lampung Selatan 35365, Indonesia
  • Sister Sianturi Program Studi Farmasi, Institut Sains dan Teknologi Nasional, Jakarta Selatan 12630, Indonesia
DOI: https://doi.org/10.35472/jsat.v3i1.111

Abstract

DNA extraction is a series of processes to separate DNA from other cell components. Extraction is the most crucial early stage in molecular research. There are two methods in DNA extraction: traditional organic extraction and non-organic adoption extraction method. Traditional organic extraction method are used by many laboratories to obtain amount of high-yield DNA. However, in recent years it has been a tendency to adopt non-organic commercial protocols, known as extraction kit. This method was relatively faster, low times-consuming and avoid the toxicity due to phenol use. This study aims to determine the steps of DNA extraction to obtain total DNA from muscle tissue of fish grouper using Extraction Kit for Animal Tissue. The Kit extraction method has an advantages that is the processing time is shorter, simple, non-toxic and high-purity DNA yields. A total of 4 tissue samples from grouper muscle were successfully extracted using an Extraction Kit that was indicated by the visualization of the band on qualitative DNA analysis in 1% agarose gel.


Keywords: DNA extraction, extraction kit, high-yield DNA, molecular method

Downloads

Download data is not yet available.

References

[1] Sambrook J, Russell DW. Molecular Cloning: A Laboratory, Third Edition. New York (US): Cold Spring Harbour Laboratory Press. 2001.
[2] Fan H, Gulley ML. Molecular Pathology Protocols: Methods in Molecular Medicine. A. A. Killeen, editor. Totowa, NJ: Humana Press Inc. 2000.
[3] Hajibabaei M, deWaard JR, Ivanova NV, Ratnasingsham S, Dooh RT, Kirk SL, Mackie PM, Hebert PDN. Critical factors for assembling a high volume of DNA barcodes. Phil Trans R Soc B. 360: 1959-1967. 2005.
[4] Albert B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Moleculer Biology of The Cell, Fifth Edition. New York (US): Garland Science, Taylor & Francis Group. 2008.
[5] Walker M and Rapley R (eds). Molecular Biomethods Handbook, 2nd edn. NJ, USA: Humana Press. 2008.
[6] Sweet D, Lorente M, Valenzuela A, Lorente JA, Alvarez JC. Increasing DNA extraction yield from saliva stains with a modified Chelex method. For Sci Int. 83: 167-177. 1996.
[7] Stefano RC, Vago L, Bonetto S, Nebuloni M, Costanzi G. Use magnetic beads for tissue DNA extraction and IS6110 Mycobacterium tuberculosis PCR. J Clin Pathol. 52: 158-163. 1999.
[8] Albertoni GA, Arnoni CP, Araujo PRB, Andrade SS, Carvalho FO, Girȧo MJBC, Schor N, Barreto JA. Magnetic bead technology for viral RNA extraction from serum in blood bank screening. Braz J Infect Dis. 15 (16): 547-552. 2011.
[9] Raffiudin R, Bintar A, Widjaja MC, Farajallah A, Purwantara B. Rapid detection of the Africanized honey bee: a tool for Indonesian animal quarantine. Biotropia. 16 (1): 38-44. 2009.
[10] Vogelstein B, Gillespie D. Preparative and analytical purification of DNA from agarose. Proc. Nat. Acad. Sci. 76: 615-619. 1979.
[11] Esser KH, Marx WH, and Lisowsky T. MaxXbond: first regeneration system for DNA binding silica matrices. Nature. 3(1): 1 – 2. 2006.
[12] Berensmeier S. Magnetic particles for the separation and purification of nucleic acids. Microbiol.Biotechnol. 73: 495-504. 2006.
[13] Mulyani Y, Purwanto A, Nurruhwati I. Perbandingan beberapa metode isolasi DNA untuk deteksi dini Koi Herves Virus (KHV) pada ikan mas (Cyprinus carpio L). Akuatika: 1-16. 2011.
[14] Glasel JA. Validity of nucleic acid purities monitored by 260 nm/280 nm absorbance ratio. BioTechniques 18(1): 62-62. 1994.
[15] Khosravinia H, Murthy HNN, Parasad DT, Pirany N. Optimizing factors influencing dna extraction from fresh whole avian blood. African J Biotechnol. 6 (4): 481-486. 2007.
Published
2019-08-31
How to Cite
ARIYANTI, Yanti; SIANTURI, Sister. Ekstraksi DNA total dari sumber jaringan hewan (Ikan Kerapu) menggunakan metode kit for animal tissue. Journal of Science and Applicative Technology, [S.l.], v. 3, n. 1, p. 40-45, aug. 2019. ISSN 2581-0545. Available at: <https://journal.itera.ac.id/index.php/jsat/article/view/111>. Date accessed: 25 apr. 2024. doi: https://doi.org/10.35472/jsat.v3i1.111.
Citation Formats
Issue
Vol 3 No 1 (2019): Journal of Science and Applicative Technology August Chapter
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License

All the content on Journal of Science and Applicative Technology (JSAT) may be used under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License.

You are free to:

  • Share - copy and redistribute the material in any medium or format
  • Adapt - remix, transform, and build upon the material

Under the following terms:

  • Attribution - You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • NonCommercial - You may not use the material for commercial purposes.
  • No additional restrictions - You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.