Penyerapan Pakan Berbahan Dasar Kacang Hijau (Vigna radiata) pada Mencit Betina (Mus musculus)
Abstract
Mencit (Mus musculus) adalah hewan model utama dalam penelitian biomedis. Kebutuhan akan pakan untuk pertumbuhan mencit mendorong pengembangan pakan alternatif dengan formulasi sesuai kebutuhan nutrisi. Salah satu bahan yang dapat digunakan adalah kacang hijau (Vigna radiata). Penelitian ini menerapkan pakan alternatif dari kacang hijau (Vigna radiata) untuk menunjang pertumbuhan mencit sebagai hewan model laboratorium dan mengamati penyerapan pakan yang diberikan. Penelitian ini bertujuan untuk membandingkan penyerapan pakan berbahan dasar kacang hijau pada berbagai variasi konsentrasi terhadap dengan pakan komersial. Perlakuan pada penelitian ini adalah kelompok kontrol (100% pakan komersial), KH85 (85% kacang hijau), KH75 (75% kacang hijau), KH65 (65% kacang hijau), dan KH55 (55% kacang hijau). Perlakuan dan pengambilan data dilakukan setiap hari selama dua bulan. Setiap mencit ditimbang sebelum dan sesudah makan untuk menentukan berapa banyak pakan yang mereka konsumsi. Mencit juga ditimbang setelah defekasi untuk menentukan berapa banyak pakan yang diserap pencernaan mencit. Berdasarkan hasil penelitian, perlakuan KH65 memiliki nilai penyerapan pakan yang tertinggi dibandingkan perlakuan lain, meskipun tidak terdapat perbedaan nyata dari pakan yang dimakan dan diserap dari seluruh perlakuan. Mencit dapat menyerap formulasi pakan berbahan dasar kacang hijau sebaik pakan komersial. Efisiensi pakan dapat dipengaruhi oleh digestibilitas bahan pakan, kebutuhan energi untuk menjaga berat badan, juga efisiensi pertumbuhan jaringan tubuh. Pada mencit betina, penyerapan pakan berbahan dasar kacang hijau setara dengan pakan komersial BR II sehingga pakan alternatif kacang hijau berpotensi menjadi pakan alternatif.
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