%A Ghifari, M Alvien %A Fachriyah, Enny %A Anam, Khairul %A Nopitasari, Dian %A Elvira, Kautsar %A Perdana, Rama Esa %D 2021 %T Identification of Alkaloid Compounds from Cytotoxic Active Fraction in Peperomia pellucida %K %X Peperomia pellucida  attracts a lot of interest due to its bioactivity and has been used in various folk medicine. In this report, n-hexane, dichloromethane, and ethyl acetate fraction of  Peperomia pellucida  extract were examined for its cytotoxic activity against  Artemia salina  larvae using the BSLT method. The ethyl acetate fraction showed higher cytotoxic activity than n-hexane and dichloromethane. The LC 50 of ethyl acetate, n-hexane, and dichloromethane fractions was 39.665, 278.920, and 60.808 ppm, respectively. Mayer and Dragendorff tests give a positive result to the alkaloid constituent in ethyl acetate fraction. The alkaloids in  Peperomia pellucida  were extracted using ethyl acetate and further separated using chromatography techniques. Two alkaloid components were identified in ethyl acetate fraction from  Peperomia pellucida  as Piperine and Nigramide N by using LC-MS/MS.  %U https://journal.itera.ac.id/index.php/jsat/article/view/394 %J Journal of Science and Applicative Technology %0 Journal Article %R 10.35472/jsat.v5i1.394 %P 208-213%V 5 %N 1 %@ 2581-0545 %8 2021-05-28